Alpha-Factor Mating Pheromone Epigenetics High-Throughput/Automated Isolation Traditional methods of sample lysis

Traditional methods of sample lysis require tedious homogenization using a pestle and mortar

is suitable for hybridization

Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range

The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR

SDS-PAGE and other sensitive applications

Alpha-Factor Mating Pheromone Epigenetics High-Throughput/Automated Isolation Traditional methods of sample lysisWhen yeast "a" and "alpha" cells encounter mating pheromones of the opposite cell type they induce genes necessary for mating, arrest the cell cycle in G1, alter cell surface and nuclear determinants, and also undergo dramatic morphological elongation into pear shapes, affectionately termed "schmooing". These alterations prepare the yeast cells for mating and fusion to form stable diploids. The a alpha diploids are not responsive to mating pheromone